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E-cigarette vapour does not induce DNA mutations commonly observed with tobacco smoke exposures in lab-based tests.
Scientists at British American Tobacco used a method called the Ames test to compare the mutagenic potential of cigarette smoke with that of vapour from Vype ePen, a commercially available e-cigarette. DNA mutations result in genetic instability, which may be involved in the development of cancer.
The Ames test is widely used method that uses bacteria to test whether a given chemical or drug causes mutations in the bacteria's DNA. The standard test involves five bacterial strains. In this study, two of these bacteria were used Salmonella typhimurium strains TA98 and TA100, both of which are effective at screening 90-95% of potential mutagens. TA98 and TA100 have been used widely to assess tobacco smoke, but never for the assessment of freshly generated e-cigarette aerosols, until now.
Traditionally, the particulate matter in smoke is assessed, but this is only a small fraction of the tobacco smoke. To more accurately reflect real-life exposure, whole smoke was also tested. In all, the researchers tested both the particulate matter and whole aerosol of smoke from a reference cigarette 3R4F and vapour from Vype ePen.
To do this, they trapped particulate matter from smoke or vapour on a filter pad and then washed the pad with a solvent to produce a stock solution that could be diluted into various concentrations. They then exposed the test bacteria to the same concentrations of either smoke or vapour extract. They also exposed test bacteria to freshly generated smoke or e-cigarette vapour.
Read more here. (EurekAlert)
Scientists at British American Tobacco used a method called the Ames test to compare the mutagenic potential of cigarette smoke with that of vapour from Vype ePen, a commercially available e-cigarette. DNA mutations result in genetic instability, which may be involved in the development of cancer.
The Ames test is widely used method that uses bacteria to test whether a given chemical or drug causes mutations in the bacteria's DNA. The standard test involves five bacterial strains. In this study, two of these bacteria were used Salmonella typhimurium strains TA98 and TA100, both of which are effective at screening 90-95% of potential mutagens. TA98 and TA100 have been used widely to assess tobacco smoke, but never for the assessment of freshly generated e-cigarette aerosols, until now.
Traditionally, the particulate matter in smoke is assessed, but this is only a small fraction of the tobacco smoke. To more accurately reflect real-life exposure, whole smoke was also tested. In all, the researchers tested both the particulate matter and whole aerosol of smoke from a reference cigarette 3R4F and vapour from Vype ePen.
To do this, they trapped particulate matter from smoke or vapour on a filter pad and then washed the pad with a solvent to produce a stock solution that could be diluted into various concentrations. They then exposed the test bacteria to the same concentrations of either smoke or vapour extract. They also exposed test bacteria to freshly generated smoke or e-cigarette vapour.
Read more here. (EurekAlert)
